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Infection of the red palm weevil, Rhynchophorus ferrugineus (coleoptera:curculionidae) by the entomopathogenic fungus Metarhizium anisopliae

Xiaodong Sun1, 2, Wei Yan1, Jing Zhang1, Xiaoqing Niu1, Fuheng Li2, Weiquan Qin1Email author and Guangchang Ma3
SpringerPlus20165:1748
DOI : 10.1186/s40064-016-3416-6© The Author(s) 2016
Received : 18 April 2016Accepted : 28 September 2016Published : 7 October 2016

Les recherches sur le Beauveria Bassiana semblent ne pas avancer suffisamment, raison sans doute pour lesquelles il faudrait s’intéresser à son cousin le Metarhizium avec 5 préoccupations :

* Efficacité plein champ au-delà de 20° et par climat sec
* Sélectivité
* Coût annuel pour une prévention efficace
* Longue rémanence plusieurs mois
* Simplicité de mise en œuvre

This study determined the pathogenicity of Metarhizium anisopliae strain SD-3 against invasive red palm weevil (RPW), Rhynchophorus ferrugineus Olivier (coleoptera:curculionidae) larvae in Hainan Province, China. Inoculation of 1 × 108 conidia/mL caused 100 % mortality of R. ferrugineus, indicating that the conidia of strain SD-3 were highly virulent. The process of invasion mechanism was showed by scanning electron microscopy (SEM) and frozen section as follows. Once R. ferrugineus was infected by strain SD-3, M. anisopliae hyphae first invaded the cuticular and body cavity of R. ferrugineus. Secondly, well-developed muscles, fat, tracheaes and digestive tube tissues in the abdomen of R. ferrugineus were then decomposed and absorbed by M. anisopliae hyphae, leading to the total destruction of the larvae. Finally, M. anisopliae hyphae reproduced, resulting in a large number of conidia in the body of RPW. The SEM and frozen section are convenient tools to observe the mode of action of entomopathogenic fungi and to observe how M. anisopliae is able to colonize it.

Frozen section and electron microscopy studies of
http://springerplus.springeropen.com/articles/10.1186/s40064-016-3416-6